Introduction: Spirulina (Arthrospira) exerts a wide spectrum of pharmacological activities that are largely attributed to its phycobiliprotein content,  mainly  to  C-phycocyanin.  C-Phycocyanin  is  a  natural  blue  pigment  with  many commercial  applications  in  foods, cosmetics, and medicines.
Methods:  In this study, the stimulatory effect of  C-phycocyanin on the immune system was investigated using blood tissue and Peripheral  Blood  Mononuclear  Cells  (PBMC)  and  the  measurement  of  inflammatory  cytokine,  interferon-gamma, which  has important effects on dedicated immune responses. For this purpose, extraction of PBMC blood cells from blood tissue The range of  purified  C-phycocyanin  extract  concentrations  with  drug  puritywas:  1  µg/ml,  10  µg/ml,  100  µg/ml,  250  µg/ml.  Then, the response of PBMC cells to C-phycocyanin at the protein level was investigated. Finally, interferon-gamma was measured using the culture supernatant and ELISA sandwich method.
Results:  Descriptive  analysis  of  the  read  concentrations  results  by  ELISA  technique  showed  that  C-phycocyanin  is dosedependent  and  the  results  of  the  effect  of  C-phycocyanin  on  PBMC  cells  in  blood  tissue  showed  the strengthening  of  the immune system by increasing the amount of inflammatory cytokines. According to the results of the analysis of variance, it isobserved that the p-value is less than 0.05. This means there is a significant difference between the mean read concentration of ELISA in different concentrations of cytokines.
Conclusion: The results of the experiments demonstrate that C-phycocyanin activates PBMC cells in a manner that is consistent with the recruitment of diverse populations of leukocytes in response to inflammatory and infectious signals.