In Silico Analysis Brucella OMPs and CagA for Expansion of a Subunit Vaccine Candidate Versus Brucellosis

Document Type: Narrative Review

Authors

1 Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

3 Chemical Injuries Research Center, System Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

4 Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran

Abstract

Brucellosis is one of the current zoonotic diseases, still a health hazard in many countries, and progressive research programs are necessary to control and eradicate this disease in endemic areas. Outer membrane proteins (OMPs) are capable of allocating immunity and protection antigen in mice. CagA is an immunogenic protein of Helicobacter pylori. Because OMPs are part of Brucella spp, they have been conferred as potential immunogenic and protective antigens. In this study, the gene sequence which encoding TN-OMPs was obtained from GenBank. To estimate the 3D structure of the protein, modeling, prediction of secondary and tertiary structure, immunogenicity, allergenic sites and antigenic B-cell and T-cell epitopes were performed. The conserved domain of proteins was obtained and the epitopes of TN-OMPs were capable of inducing both B-cell and T-cell mediated immune responses. CagA is a cellular immunogenic protein that induces a Th1 response. The OMPs of Brucella could be used as a suitable vaccine candidate versus brucellosis.

Keywords


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