Vaginismus as a common sexual disorder among women affects on approximately 1-7% of females in a worldwide. There is variety in etiology and diagnosis. In addition, classification of vaginismus and clarify the level would be useful in effective treatment. Vaginismus currently classified as Genito-Pelvic Pain / Penetration Disorder in latest edition of Diagnostic and Statistical Manual of Mental Disorders, also known as vaginal penetration disorder. It is involuntary and uncontrolled and functions much the same as any reflex to avoid injury. It is the most common reason for unconsummated marriages. In this review, we present different etiologies and updated techniques for diagnosis and treatment. Newly use of Botox as a logical treatment for vaginismus had been proposed and in this literature, we express recent research and data.